Journal: Medicine

Article Title: Clinical evaluation of the loop-mediated isothermal amplification assay for the detection of common lower respiratory pathogens in patients with respiratory symptoms

doi: 10.1097/MD.0000000000013660

Figure Lengend Snippet: Schematic of the microfluidic chip consisting of an array of 24 reaction wells and microchannel for sample inlet, distribution and reaction. (A) schematic drawing of microfluidic chip and (B) photograph.

Article Snippet: The reactions were performed on a RTisochipTM-A thermostatic expansion microfluidic chip nucleic acid analyzer (Fig. B), along with the real-time imaging system ((Fig. B)(CapitalBio Technology, Beijing, China), according to the following protocol: 1 cycles of 3 minutes at 37°C and 1 cycles of 47 minutes at 65°C.

Techniques:

Journal: Medicine

Article Title: Clinical evaluation of the loop-mediated isothermal amplification assay for the detection of common lower respiratory pathogens in patients with respiratory symptoms

doi: 10.1097/MD.0000000000013660

Figure Lengend Snippet: Microcentrifuge and setup CapitalBio RTisochip. (A) Microcentrifuge that used to make the mixture drop into the bottom of the reaction wells. (B) CapitalBio RTisochip-A equipped with a temperature-controlled system and a real-time imaging system. (C) Amplification curves for respiratory pathogens detection obtained using the microfluidic chip.

Article Snippet: The reactions were performed on a RTisochipTM-A thermostatic expansion microfluidic chip nucleic acid analyzer (Fig. B), along with the real-time imaging system ((Fig. B)(CapitalBio Technology, Beijing, China), according to the following protocol: 1 cycles of 3 minutes at 37°C and 1 cycles of 47 minutes at 65°C.

Techniques: Imaging, Amplification